Table 4 Multivariate analysis for semen quality characteristics of fresh and frozen-thawed semen and elements Fe, Se and Zn measured in blood serum on the day of collection

Fresh semen

 Motility^a

80,738 (−39,156 to 2,00,632)

8,02,538 (4,61,390–1,143,685)

94,064 (39,659–1,48,469)

 Progressive motility^a

82,652 (−20,842 to 1,86,147)

6,49,692 (3,55,208–9,44,176)

44,795 (−2169 to 91,758)

 Viability^a

1,23,403 (−7048 to 2,53,853)

7,82,244 (4,11,058–1,153,430)

47,977 (−11,218 to 1,07,173)

 Normal morphology^a

−5386 (−1,21,992 to 1,11,220)

6,08,177 (2,76,385–9,39,969)

1,03,614 (50,701–1,56,528)

 Acrosome defects^b

−0.6213 (−1.3876 to 0.1451)

−0.7251 (−2.9057 to 1.4555)

−0.4135 (−0.7613 to −0.0658)

 Normal tail membrane integrity^a

−85,817 (−2,25,635 to 54,001)

5,05,314 (1,07,474–9,03,153)

70,115 (6669–1,33,561)

 DNA fragmentation

−3.5201 (−6.4732 to −0.5670)

−6.2227 (−14.6255 to 2.1802)

−1.0848 (−2.4249 to 0.2552)

Frozen-thawed semen

 Motility^a

38,919 (−75,091 to 1,52,930)

7,81,556 (4,57,150–1,105,962)

71,343 (19,608–123,078)

 Progressive motility^a

4328 (−65,223 to 73,878)

2,33,317 (35,418–4,31,216)

52,522 (20,962–84,083)

 Viability^a

67,129 (−72,219 to 2,06,477)

6,34,447 (2,37,944–1,030,950)

95,076 (31,843–1,58,309)

 Normal morphology^a

−59,182 (−1,73,925 to 55,561)

5,48,513 (2,22,021–8,75,005)

91,924 (39,856–1,43,992)

 Acrosome defects^b

0.0237 (−0.4278 to 0.4752)

−1.2739 (−2.5585 to 0.0107)

−0.1664 (−0.3713 to 0.0385)

 Normal tail membrane integrity^a

−19,935 (−1,43,509 to 1,03,638)

8,14,528 (4,62,911–1,166,146)

47,923 (−8152 to 1,03,998)

 DNA fragmentation

−1.5635 (−5.6921 to 2.5652)

−13.9299 (−25.6775 to −2.1822)

−2.4675 (−4.3410 to −0.5940)