Table 1 Precision and accuracy tests of the canine CRP ICA using serial dilutions of four serum samples. The CRP levels were determined with our ELISA at a 1:1000 sample dilution. ICA results: −, negative; ± , weak positive; and +, positive
Sample # | Dilution | CRP (μg/mL) | ICA results |
|---|---|---|---|
S1 | 1:2 | 38.2 | + |
1:4 | 19.0 | + | |
1:8 | 9.3 | + | |
1:16 | 4.4 | ± | |
1:32 | 2.0 | − | |
1:64 | 0.9 | − | |
S2 | 1:2 | 29.4 | + |
1:4 | 14.4 | + | |
1:8 | 7.0 | ± | |
1:16 | 3.5 | − | |
1:32 | 1.6 | − | |
S3 | 1:2 | 18.9 | + |
1:4 | 9.1 | ± | |
1:8 | 4.3 | − | |
1:16 | 2.1 | − | |
S4 | 1:2 | 56.6 | + |
1:4 | 29.2 | + | |
1:8 | 14.8 | + | |
1:16 | 6.9 | ± | |
1:32 | 3.4 | − | |
1:64 | 1.7 | − |