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Fig. 7 | Acta Veterinaria Scandinavica

Fig. 7

From: Age-related changes in the shell gland and duodenum in relation to shell quality and bone strength in commercial laying hen hybrids

Fig. 7

Oestrogen receptor β (ERβ) immunolocalization in shell gland and duodenum of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) hens at different ages during a production period. a Shell gland mucosal fold of 49-week-old LSL hen. Strong nuclear staining for ERβ in non-ciliated cells of the surface epithelium (S). Weakly stained or negative nuclei in ciliated cells. Tubular gland cells (G) show strong nuclear staining for ERβ. Weak to moderate cytosolic staining in surface epithelium (S) and tubular gland cells (G). Lumen (L), bar = 20 µm. b Duodenum of 21-week-old LSL hen showing moderate to weak nuclear staining for ERβ in surface epithelium of villi (V), crypts of Lieberkühn (Cr) and smooth muscle cells (M). Weak cytosolic staining for ERβ in the surface epithelium (S). The staining gradually decreases towards the top of the villi and most of the nuclei at the top are negative. Lumen (L), bar = 100 µm. Weak haematoxylin counterstain in both images

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