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Table 1 The quantitative real-time PCR primers for DIO1 and two housekeeping genes in the liver biopsy specimens of pregnant and non-pregnant heifers on day 18

From: Peripheral thyroid hormone levels and hepatic thyroid hormone deiodinase gene expression in dairy heifers on the day of ovulation and during the early peri-implantation period

Gene symbol

Primer

Primer sequence (5′→3′)

Accession number

Start

Product bp

GAPDH

Forward

caa cat caa gtg ggg tga tg

NM_001034034.1

315

202

Reverse

ggc att gct gac aat ctt ga

516

RPS9

Forward

gat tac atc ctg ggc ctg aa

NM_001101152

340

201

Reverse

cag gga gaa gtc gat gtg ct

540

DIO1

Forward

ccg tgg tgg tag aca caa tg

NM_001122593.1

605

204

Reverse

tca ggt tgg gca cct aga ac

808

  1. GAPDH glyceraldehyde-3-phosphate dehydrogenase; RPS9 ribosomal protein S9; DIO1 deiodinase-1