Optimization and evaluation of Flexicult® Vet for detection, identification and antimicrobial susceptibility testing of bacterial uropathogens in small animal veterinary practice

Table 3 Growth of 15 methicillin-resistant (MRSP) and 15 methicillin-susceptible Staphylococcus pseudintermedius (MSSP) strains on Flexicult Vet agar base supplemented with different oxacillin concentrations (µg/ml) in the presence (+) or absence (–) of 2 % NaCl

Oxacillin conc. (µg/ml)	2 % NaCl	Incubation time (h)	MRSP growth/no. of isolates	MSSP growth/no. of isolates
0.5	–	24/48^a	7/15	0/15
	+	24	9/15	0/15
	+	48	10/15	0/15
0.25	–	24/48^a	10/15	0/15
	+	24	10/15	0/15
	+	48	11/15	0/15
0.125^b	–	24/48^a	13/15	0/15
0.125^b	+	24/48^a	15/15	0/15
0.062	–	24/48^a	15/15	15/15
0.062	+	24/48^a	15/15	15/15

The results are presented as the proportions of correctly classified MRSP (growth) and MSSP (no growth) isolates following 24 and 48 h of incubation
^aThe same result was recorded after 24 and 48 h of incubation
^b0.125 µg/ml oxacillin + 2 % NaCl was selected for Flexicult Vet B replacing the cephalotin compartment in Flexicult Vet A

ISSN: 1751-0147