Table 1 Monoclonal antibodies tested
Antibody | Target | Host | Isotype | Clone | Manufacturer | Catalog | Reactivity |
|---|---|---|---|---|---|---|---|
Anti-IL-1α | Human | Mouse | IgG1 | 3643B314 | BD | 554561 | + |
Anti-IL-1β | Human | Mouse | IgG1 | AS10 | BD | 340516 | – |
Anti-IL-2 | Human | Mouse | IgG1 | 5344.111 | BD | 340450 | – |
Anti-IL-4 | Human | Mouse | IgG1 | 8F-12 | IQProducts | IQP162R | + |
Anti-IL-5 | Human | Rat | IgG2 | JES139D10 | BD | 559332 | + |
Anti-IL-6 | Human | Rat | IgG1 | MQ2-13A5 | BD | 554545 | + |
Anti-IL-8 (#1) | Human | Mouse | IgG2 | G265-8 | BD | 554720 | + |
Anti-IL-8 (#2) | Human | Mouse | IgG1 | E8N1 | Biolegend | 511408 | + |
Anti-IL-10 | Human | Rat | IgG1 | JES3-16E3 | BD | 554467 | – |
Anti-IL-12 | Human | Mouse | IgG1 | C11.5 | BD | 554575 | + |
Anti-IL-13 | Human | Rat | IgG1 | JES10-5A2 | BD | 559328 | – |
Anti-IL-17A | Human | Mouse | IgG1 | eBio64DEC17b | eBioscience | 12-7179-73 | + |
Anti-IFN-γ(#1) | Human | Mouse | IgG1 | B27 | BD | 559327 | – |
Anti-IFN-γ(#2) | Human | Mouse | IgG1 | 45-15 | IQProducts | IQP160r | – |
Anti-TNF-α(#1) | Human | Mouse | IgG1 | B-C7 | IQProducts | IQP163R | + |
Anti-TNF-α(#2) | Human | Mouse | IgG1 | mab11 | BD | 559321 | + |
Anti-TGF-β | Human | Mouse | IgG1 | TB21 | IQProducts | IQP169R | + |
Isotype control | – | Mouse | IgG1 | – | BD | 555748 | – |
Isotype control | – | Mouse | IgG2 | – | BD | 556437 | – |
Isotype control | – | Rat | IgG1 | – | BD | 554685 | – |
Isotype control | – | Rat | IgG2 | – | BD | 557229 | – |
Anti-IL-4 | Bovine | Mouse | IgG2a | CC303 | Serotec | mca1820pe | + |
Anti-IFN-γ | Bovine | Mouse | IgG1 | CC302 | Serotec | mca1783pe | + |