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Fig. 2 | Acta Veterinaria Scandinavica

Fig. 2

From: Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining

Fig. 2

Cross-reactivity of a range of anti-human cytokine mAbs with canine cytokines following short-term whole blood cultures in vitro. Dot plot distribution graphs of empty channel (FL4 fluorescence) versus PE-emission channel (FL2) were used to quantify the percentage of cytokine+ lymphocytes in unstimulated controls (left panels) and PMA + LPS stimulated cultures (right panels). Only Q1 and Q4 quadrants are represented. Gray background highlights the reactivity above 0.5 % in the quadrant Q1

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