- Open Access
Epidemiology of methicillin resistant Staphylococcus pseudintermedius in guide dogs in Finland
Acta Veterinaria Scandinavica volume 57, Article number: 37 (2015)
Methicillin resistant Staphylococcus pseudintermedius (MRSP) and Staphylococcus aureus (MRSA) are common multi-drug resistant (MDR) bacteria in dogs. In 2012–2013 three dogs of the Guide Dog School of the Finnish Federation of the Visually Impaired were found to be MRSP positive. Guide dogs have regular contact with each other during their first year of life and prolonged contact when in training. Since dogs are placed in different parts of Finland after training, there is a risk for national spread of MDR bacteria. In this study the prevalence of MRSP and MRSA, as well as the risk factors for MRSP were determined in the Finnish guide dog population. MRSP isolates were investigated using molecular methods and compared to the earlier isolates.
Out of 132 tested dogs 4 were MRSP positive thus giving the prevalence estimate of 3% (95% CI: 1–8%) for MRSP in the target population. MRSA was not detected (prevalence estimate 0%, 95% CI: 0–3%). Risk factors associated with MRSP were being a breeding bitch (OR = 8.4; 95% CI: 1.1–64.1, P = 0.012), the number of veterinary visits (OR = 1.23; 95% CI: 1.0–1.5, P = 0.025) and number of antimicrobial courses (OR = 1.63; 95% CI: 1.0–2.55; P = 0.035). Identified MRSP isolates belonged to five different sequence types (ST45, 71, 402, 403 and 404). All ST71 isolates carried SCCmec II-III, while the SCCmec type of the ST45 and ST402 (a single locus variant of ST45) isolates were non-typeable with the method used.
MRSP and MRSA had low prevalence in the studied dog population despite the close contact between dogs, and the MRSP population was heterogenic. Antimicrobial therapy and veterinary visits are risk factors for MRSP even among a small case group.
Staphylococcus pseudintermedius belongs to the normal microbiota of dogs and is an opportunistic pathogen . The bacterium can cause a plethora of infections in dogs and is also capable of causing infections in humans . Since the mid 2000’s methicillin resistant S. pseudintermedius (MRSP) isolates have become more common [3, 4]. This has raised concern of emerging antimicrobial resistance and infections that are difficult to cure with available antimicrobials . MRSP has spread clonally in Europe and North America . The predominant European clone, ST71(MLST)-t02(spa)-II-III(SCCmec), has been determined to be the principal MRSP clone in Sweden  and has also been observed in Finland, where it caused a large outbreak in a veterinary teaching hospital . Of other staphylococci, Staphylococcus aureus is a common human pathogen, but is less prevalent in dogs . Nevertheless, methicillin resistant S. aureus (MRSA), traditionally a nosocomial pathogen of humans, is also found in dogs and can cause infections in them . MRSA may also be more readily transmitted between dogs and owners than MRSP [10, 11].
The Guide Dog School of the Finnish Federation of the Visually Impaired provides guide dogs to the blind and visually impaired in Finland. In 2012–2013, MRSP was found in routine clinical specimens of three guide dogs, all suffering from atopic dermatitis. The dogs of the Guide Dog School constitute a special group of animals; they are in close contact during training at the Guide Dog School kennel, after which they are sent all over the country. During their service years, they also regularly visit the school’s premises. Thus nationwide risk for spread of resistant bacteria is a valid concern. Due to these factors it was decided that the epidemiology of MRSP and MRSA needed to be determined in this population. The present study was conducted (1) to establish the prevalence of MRSP and MRSA in the Finnish guide dog population, (2) to genotype and compare isolates from the Guide Dog School’s dog population and (3) to detect risk factors for carriage of MRSP.
This study consisted of four parts; (1) a cross-sectional prevalence study of MRSP/A in the guide dog population as well as a case–control study to determine the risk factors for MRSP/A, (2) the screening of contact dogs of MRSP positive dogs from the prevalence study, along with extended screening of breeding bitches of the Guide Dog School and (3) the comparison of the MRSP isolates from these dogs. The fourth part of the study included mapping of possible transmission routes as well as longitudinal follow-up of the MRSP-status of four dogs.
The prevalence and risk factor study
The guide dog population
The Guide Dog School (GDS) of the Finnish Federation of the Visually Impaired is situated in the greater Helsinki area in Finland . The school is a nonprofit organization whose mission is to enhance the independence of people who are blind or visually impaired through the use of specially trained dogs. The school breeds the majority of their dogs themselves. Breeding bitches are housed in volunteer families. The bitches give birth at the school’s facilities in a separate ward reserved for this purpose. Puppies spend their first 6 weeks with their dam at the school after which they are weaned and housed in volunteer families. During the first year of their life the dogs have regular (short) appointments at the school. At the age of 13–18 months guide dog candidates are transferred back to the school where they undergo suitability testing. If selected, the dog begins a training period of 20 weeks after which it is placed for service as a guide dog. During their service the dogs spend short periods at the school in training; the GDS’s facilities also serve as a kennel to guide dogs during vacations of their host/hostess. At the time the study was planned, the size of the guide dog population was approximately 330 dogs. Out of these roughly 110 dogs were in training, 200 worked as guide dogs and 20 animals were breeding bitches.
Sample size calculation
Sample size for the entire 330 dog population was calculated using EpiTools . The estimated sample size was 152 dogs with an approximated MRSP prevalence of 3% with a ±2% desired precision on a 95% confidence level. The occurrence of MRSP varies significantly between studies depending on used methods, population and geographical origin. Figures between zero and 42 percent have been reported [14–21]. The guide dogs were considered a low risk population, thus the three percent estimate was based on studies that were assessed to represent our target population. These showed a prevalence between 1.5 and 4.5 percent [15–17, 20, 21]. The calculation of sample size for MRSA separately was not considered necessary, since the prevalence of MRSA was expected to be less than that of MRSP.
Specimens and data collection
Specimens for the prevalence study were collected between February and November 2014 when the dogs visited the GDS. This method was selected out of convenience instead of random sampling. Three to four sites of each dog were swabbed by using sterile cotton tips (M40 Transsystem Amies Agar Gel without charcoal, Copan Diagnostics Inc., Italy): the first swab was taken from the mucous membranes of the anterior nares and mouth (combined swab), the second swab from the perineal area, and the third from skin abrasions/wounds if present. The specimens were then transferred to the Clinical Microbiology Laboratory at the Faculty of Veterinary Medicine of the University of Helsinki. In the laboratory the specimens were kept refrigerated at 4°C if they arrived outside office hours. All specimens were processed within 2 days of sampling.
To determine the representativeness of the sample population to the target population, the age, sex, breed and dog group variables as well as the exact number of dogs in the whole population and different subgroups were collected from the entire target population. To identify possible risk factors for MRSP, the following data were collected from the dogs of the sample population: breed, sex, age, whether the animal was bred by the GDS or purchased, whether it was a trainee, at service or a breeding bitch, and medical history. Medical history included information on previous skin disease, antimicrobial and other medical treatments within a 12 month period prior to entering the study.
The prevalence estimates for MRSP and MRSA were calculated by number of positive specimens divided by total number of collected specimens and presented as percentages. The 95% confidence intervals (95% CI) for the prevalence estimates were determined using an EpiTools calculator . Confidence intervals were reported based on the Wilson score interval due to the low prevalence . The representativeness of the sample population was assessed by using the Fisher’s exact test for sex, breed and dog group, and using an independent two-sample t test for age.
Descriptive statistics of the study variables were calculated by MRSP status. To identify risk-factors, the associations of the studied variables (see “Specimen and data collection”) with a positive MRSP result were evaluated with univariable logistic regression models. Due to very few positive MRSP results in the data, the rareness of the events was taken into account in the modelling by applying Firth’s bias adjustment method , which maximizes a penalized likelihood function, instead of the standard maximum likelihood function. Odds ratios (OR) with 95% profile likelihood  confidence intervals (CI) were calculated to quantify the results. P values ≤0.05 were considered statistically significant. All statistical analyses were done using SAS® System for Windows, version 9.3 (SAS Institute Inc., Cary, NC, USA).
Specimens from contact dogs and breeding bitches
Three contact dogs of MRSP positive guide dogs were screened for MRSP. Because two breeding bitches were observed to be MRSP-positive in the prevalence study, MRSP screening was extended to the rest of dogs of that subpopulation (n = 5). The same technique as for the prevalence study was used when taking these specimens.
Mapping of transmission routes, and follow-up specimens
Information on possible transmission routes was obtained by questioning the caregiver of the dogs as well as from event logs of the guide dog school. The MRSP statuses of four dogs were followed for over 6 months. Specimens were collected as described above. MRSP decolonization therapy using chlorhexidine washes and/or topical application of fusidic acid to mucous membranes was attempted in three of these dogs.
Culture, identification and susceptibility testing
Specimens from the same dog were pooled into an enrichment broth (Tryptic Soy Broth supplemented with 6.5% NaCl, Tammer-Tutkan Maljat, Finland) and incubated for 16–22 h at +35.0°C. Then an aliquot of the broth was plated onto a selective agar plate (MRSA Select, BioRad, USA). Plates were incubated up to 48 h at +35.0°C and were inspected daily. Species identification was done by conventional means; as described by Grönthal et al. 
Antimicrobial susceptibility was tested by the CLSI disk diffusion method to the following antimicrobials: oxacillin, erythromycin, clindamycin, tetracycline, sulfamethoxazole-trimethoprim, fusidic acid, enrofloxacin, gentamicin and amikacin [26, 27]. SIR categories were assigned based on the breakpoints of the CLSI standard, except for fusidic acid, for which breakpoints of ≥24 mm (susceptible) and ≤18 mm (resistant) were used . Staphylococcus aureus ATCC 25923 was used as a quality control strain for susceptibility testing.
Molecular typing of the MRSP isolates
Molecular typing was performed for MRSP isolates detected in the prevalence study, MRSP isolates of the contact dogs and for previous MRSP isolates from the guide dogs.
To detect clonal clusters, mecA-positive isolates were typed using pulsed-field gel electrophoresis (PFGE)  with modifications . All isolates were analyzed with both SmaI and AscI restriction enzymes. DNA fragments were separated on 1% agarose by using a Chef DR III system (Bio-Rad, USA). The total run time was 22 h; the first-block switch time was 0.1–15 s for 15 h, and the second-block switch time was 15–60 s for 7 h. The voltage for the run was 6 V/cm with an included angle of 120°. PFGE patterns were analyzed using Gel Compar II software (version 6.6, Applied Maths, Belgium). UPGMA based cluster analysis with the Dice similarity coefficient was used with optimization and position tolerance both set at 1%. Isolates were clustered using an 85% similarity cut-off.
DNA for mecA-PCR, SCCmec and MLST-typing was extracted using a commercial kit (InstaGene Matrix, Bio-Rad, USA) as previously described . Oxacillin resistant S. pseudintermedius isolates were confirmed to carry the mecA-gene using PCR-primers (mecA P4 and mecA P7) described by Stegger et al. . Both primers had a concentration of 0.25 µM in a final reaction volume of 20 µl. The PCR was performed with a BioRad CFX96 Real-Time PCR detection system using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA) with initial denaturation at 98.0°C for 2 min, followed by 40 cycles of denaturation (98.0°C for 5 s) and annealing/elongation (60.0°C for 45 s). Finally a denaturation step (98.0°C for 5 s) preceded the melt-curve analysis (65.0°C to 95.0°C in 0.5°C increments) that was used to verify the product. The product had a melting point of 77.5–78.0°C. Staphylococcus aureus ATCC 43300 was used as a positive control for mecA-testing.
The SCCmec cassettes were typed using a previously described multiplex PCR-method  with the following modifications: In M-PCR-1 primers γR and γF had a final concentration of 0.3 µM, while all other primers in M-PCR-1 and M-PCR-2 had a final concentration of 0.2 µM. The PCRs were performed with Phire Green HotStart II DNA polymerase (Thermo Scientific, USA) with 200 µM of each dNTP in a reaction. The conditions for the PCRs were as follows: Initial denaturation at 98.0°C for 1 min, followed by 40 cycles of denaturation (98.0°C for 15 s), annealing (56.0°C for 10 s) and elongation (72.0°C for 45 s), and a final elongation for 2 min at 72.0°C. Bands were visualized using SYBR Safe DNA stain (Life Technologies, USA) after electrophoresis in a 1% agarose.
MLST typing was performed according to the method described by Solyman et al.  with modifications: Phusion Flash High-Fidelity PCR Master Mix (Thermo Scientific, USA) was used for the reaction; the tuf-primers had a final concentration of 0.375 µM each, while all other primers had a concentration of 0.25 µM. The PCR-protocol consisted of a 15 s initial denaturation at 98°C, 30 cycles of denaturation (98°C for 2 s), annealing (52°C for 10 s) and elongation (72°C for 15 s), with final extension for 1 min at 72°C. Each PCR-product was purified using Exonuclease I (Thermo Scientific, USA) and FastAP thermosensitive alkaline phosphatase (Thermo Scientific, USA) according to the manufacturer’s instructions. The sequencing for MLST was performed at a commercial laboratory (Macrogen Inc., Netherlands) with an ABI 3730 XL automated sequencer. The sequences were analyzed using the CLC Main Workbench (version 6.9.1, CLC bio, Denmark) with CLC MLST module (version 1.4.7, CLC bio, Denmark) comparing sequences of the housekeeping genes to the S. pseudintermedius MLST database .
Prevalence and risk factor study
Specimens were taken from 132 dogs of which four were MRSP-positive (prevalence estimate for the population 3%, 95% CI: 1–8%). None of the screened dogs were positive for MRSA (prevalence estimate 0%, 95% CI: 0–3%). The breed and sex of the sample population was representative to the target population, while dog groups and age differed to that of the target population (Table 1).
Skin or ear disease history was reported in 49% of the dogs (range 43–55%; lowest in trainees, highest in guide dogs) and antimicrobial treatment in 52% of the dogs (range 43–60%; lowest in guide dogs, highest in trainees). Logistic regression analyses revealed three risk factors; (1) being a breeding bitch, (2) the number of antimicrobial courses received and (3) the number of veterinary visits. Results from logistic regression are presented in Table 2.
Screening of contact dogs and breeding bitches
Three contact dogs were screened of which two were MRSP positive. These both (dogs P-853 and 2014-887) were living together with the guide dog P-833. The contact P-853 gave a negative result in the first screening, but was observed to be MRSP positive in the second screening a month later (Figure 1). Extended screening of the rest of the breeding bitches (n = 5) revealed no cases of MRSP.
Comparison of MRSP isolates
Characteristics of the nine MRSP isolates are compared in Figure 2: four were from the prevalence study (P-833, P-834, P-843 and P-860), two (P-853 and 2014-887) were from contact dogs of the guide dog P-833 and three were isolates that had been preserved in 2012–2013 from other guide dogs (P-495, P-527 and P-781). All but one isolate (P-781) were multiresistant (resistant to ≥3 antimicrobial groups). The MRSP isolate of a contact dog 2014-887 shared an identical antibiogram with the MRSP of the guide dog P-833, but the other contact’s isolate (P-853) differed from these (Figure 2).
All, except the isolate 2014-887, were available for molecular typing. Of the four isolates from the prevalence study, three (P-833, P-843 and P-860) were non-typeable by SmaI restriction but gave identical AscI-restriction profiles (Additional file 2). Both SmaI and AscI restriction patterns indicated that the other five isolates were not closely related (Figure 2; Additional File 2).
The four MRSP-isolates from the prevalence study represented three different sequence types (Figure 2). Two were of ST45, while ST71 and ST402 had one representative each. The ST402 strain was a single locus variant of ST45: it had an A166 to G substitution in the purA 2 allele sequence compared to ST45. The new purA allele number was 40 . The isolate from dog P-781 was assigned a new sequence type (ST403) being closest to ST41, with a different cpn60 allele type (13 instead of 25). The isolate from dog P-495 was closest to ST150, but had a different fdh allele type (4 instead of 1) and a G6 to A substitution in the sar 1 allele (assigned sar allele number 17). This isolate was designated sequence type ST404 . Isolates belonging to ST71 harbored SCCmec II-III. The ST45 and ST402 isolates were non-typeable by the SCCmec method used, as only the mecA-gene was amplified in M-PCR-1. Isolate P-781 that belonged to ST403 gave an unusual result in the SCCmec analysis as it seemed products specific to gene allele’s ccrA2 and ccrA4 were amplified in M-PCR-1 (Additional file 1).
Investigation of transmission routes
Transmission route investigation revealed that dogs P-843 and P-860 had been in direct contact at the GDS. Also, dogs 2014-887 and P-853 lived in the same family as dog P-833. A member of this family worked at a small animal practice, where the dogs had visited. No other apparent temporal or spatial connections between the dogs were found.
Samples were collected from four MRSP positive dogs. MRSP decolonization therapy was performed on three dogs of which two were guide dogs (P-833 and P-844) and one was a contact of the guide dog P-833. The other contact of dog P-833 was euthanized due to severe illness. The dogs were followed for over 6 months, with four to seven samples taken roughly 2 weeks to 3 months apart. The timing and results of the follow-up samples are presented in Figure 1.
The prevalence of MRSP was determined to be 3% in the Finnish Guide dog population, matching the predicted prevalence. No MRSA positive dogs were discovered, but the true prevalence lies somewhere between 0 and 3%. There are some study limitations to consider. As seen in Table 1, dogs in training were overrepresented at the expense of guide dogs. This discrepancy is explainable by ease of access, as dogs in training were more readily available for sampling at the GDS. This is also a reason to the difference in age, since dogs in training are younger than other dogs. The sample population represented the target population well as for breed and sex, and the targeted sample size (152) was close to the obtained (132). The fact that the sample population was slightly skewed could have impacted the results of the study. Also, as previously reported , the screening method used in this study has a limit of detection of ~10 colony forming units for MRSP with a oxacillin minimum inhibitory concentration (MIC) ≥4 µg/ml. Therefore, using this method, MRSPs with very low oxacillin MIC may be missed, and thus the prevalence may be slightly underestimated. As this was a known problem, pure cultures were readily taken from selective plates upon the slightest indication of a suspected colony. In our experience this has yielded many carriers with MRSP-isolates having low oxacillin-MIC (2 µg/ml). In this study a large portion (49%) of the sampled dogs did have a history of skin or ear disease which is a well-known risk factor for MRSP [7, 35]. This potentially increased the likelihood for the presence of MRSP/A carriers in the population and subsequently compensated possible underestimation of the prevalence caused by our screening method. In all, the discrepancies in representativeness and methodology were in our opinion fairly minor and did not significantly impact the result of the study.
Published studies for the prevalence of MRSP or MRSA in Finnish dogs are not available. However, to give some contrast; there were 581 MRSP/A screening specimens investigated in our laboratory in 2014. MRSP was found in 56 of these (9.6%) while MRSA was only found in 5 specimens (0.9%). It is important to note that these specimens were taken from dogs with identifiable risk factors or patients that had been exposed to MRSP or MRSA. Only a few studies exist that have measured the prevalence of MRSP among specific animal groups. In a study of shelter animals by Gingrich et al.  in Colorado, USA, the prevalence of MRSP in dogs was 3%, while the prevalence of MRSA in dogs was 0.5%. These numbers coincide quite well with our study. In Slovenia, the prevalence of MRSP (then MRSI) was 1.5% (3/200) in clinically healthy dogs in a community, while MRSA was not discovered . Regarding veterinary hospitals, Hanselman et al.  reported a MRSP prevalence of 2.1% and MRSA prevalence of 0.5% in dogs upon admission in Ontario, Canada. A similar study conducted in Hannover, Germany reported a higher MRSP prevalence (7.4%) . Further, Boost et al.  investigated the prevalence of S. aureus in dogs and their owners in Hong Kong. They found an MRSA prevalence of 0.7% among the dogs investigated, which falls into the 0-3% prevalence estimate in our study and corresponds well to our laboratory data. Further study is however needed to ascertain the prevalence of MRSP and MRSA in the average dog population as well as subpopulations, to identify target groups for preventive measures.
In this study, a dog had a higher risk for MRSP if it was a breeding bitch or had a greater number of antibiotic courses or veterinary visits, but due to the low number of MRSP positive cases our results are only supportive at best. The fact that being a breeding bitch was significant in the risk factor analysis is likely a coincidence due to the low number of dogs in this group. As Figure 1 indicates, one of the MRSP positive breeding bitches (P-860) was only a transient carrier and had likely caught it through contact to another MRSP positive dog (P-843). Extended screening of the breeding bitches did not reveal any new MRSP cases, indicating that it is not a problem among these dogs. It would however be preferable if breeding bitches would remain MRSP negative to avoid the transfer of these bacteria to new generations of guide dogs. Further, there are apparent dependencies between some risk factors: for example, dogs with multiple antimicrobial courses have visited a veterinarian more often. Controlling of these types of confounders with multivariable modelling was not possible in our study due to very small number of MRSP positive dogs. Thus far, there is however compelling evidence to suggest that antimicrobial therapy is a major risk factor for acquiring MRSP/A [7, 36, 37]. Other, previously identified, risk factors for MRSP include hospitalization [35, 36] and repeated veterinary visits [35, 36]. Glucocorticoid therapy has also been implicated as a risk factor for MRSP by some studies [35, 36].
A considerable number (52%) of dogs in this study had received antimicrobial therapy in the twelve months prior to the study. Such a large portion of animals receiving antimicrobials is concerning. Much of the use is likely explained by the large proportion (49%) of dogs having history of skin or ear disease of some sort. Labrador retrievers, which constitute the vast majority of the guide dog population, are known to be predisposed to atopy , which exposes them to skin and ear infections and subsequent use of antimicrobials. National, species specific antimicrobial consumption data are not readily available for Finland. In Sweden however, such data has been published in the SVARM report . The amount of oral antimicrobials prescribed per dog corresponded to 563 packages per 1000 dogs in 2009 in Sweden. While many dogs likely received multiple packages that year, the number is still indicative of a high rate of antimicrobial use, comparable to our data. . The use of antimicrobials in dogs could be reduced by prioritizing a healthy skin in breeding choices, which is already part of the GDS’s breeding program.
The MRSP isolates of this study were rather heterogeneous. For example; the isolates belonging to the same clonal lineage, ST71, had different PFGE and antibiogram profiles. This suggests different sources of contagion, possibly outside of the guide dog population. This was somewhat surprising, considering the potential for clonal spread in a kennel environment. It is possible that the MRSP population in Finland is becoming more heterogeneous, perhaps due to a decrease in ST71 occurrence, as has been seen in Sweden . Only two of the dogs (P-843 and P-860) were reported to have been in contact with each other. They both carried ST45 with identical antibiogram and AscI PFGE-pulsotype. ST71 and ST45 have previously been found in Finland , but three new sequence types (STs 402, 403 and 404) were discovered in this study (Figure 2). The ST403 had a very surprising antibiogram, being resistant only to oxacillin and fusidic acid. In our experience such MRSP isolates are very rare. Osland et al.  described MRSP isolates with a similar antibiogram that belonged to ST127. This clone differs in four out of seven MLST loci from our ST403  suggesting a lack of any clonal relationship. Also, the variability of the antibiograms in the ST71 isolates was novel, as one of the three isolates displayed resistance to all antimicrobials but aminoglycosides, while others were susceptible to tetracycline and fusidic acid and amikacin, but resistant to gentamicin. Antibiograms of the ST71 isolates of this study also differed from the ST71 strain causing our veterinary teaching hospital outbreak . The outbreak strain was susceptible only to amikacin and fucidic acid. Based on our unpublished observations, this study, and a previous one by Grönthal et al. , ST45 seems to be a noteworthy MRSP-clone in Finland. However, ST45 is not commonly reported in neighboring countries, such as Sweden  or Norway , nor has it been purported as a major clone on a more global scale . It has however been reported as a major clone in Thailand and Israel .
MRSP isolates that are not typeable by SmaI PFGE and SCCmec, have been described in the Netherlands , Thailand and Israel , and Australia . The isolates that were non-typeable by SCCmec in the study by Laarhoven et al.  belonged to MRSP STs 29, 111, 131 and 143, while only ST29 was non-typeable by SmaI PFGE. The allelic profiles of these differ from that of ST45. The ST45 and other STs belonging to the clonal complex CC45 harbor a pseudo SCCmec element (ΨSCCmec 57395) described by Perreten et al. . The ST45 and ST402 MRSPs isolated in this study will be investigated further in the future to confirm the presence or absence of the pseudo SCCmec element.
Based on the results form PFGE, MLST and SCCmec analyses, as well as transmission route investigation it seems clear that dogs P-843 and P-860 carried the same MRSP-type. Dog P-833, whose MRSP isolate belonged to ST402 had two contact dogs; P-853 and 2014-887. While the MRSP isolate for dog 2014-887 was not available for further study, it did display an identical antibiogram with isolates belonging to ST45/402 indicating that the isolate also belongs to one of these sequence types. MRSP isolates from the same household are usually similar  but some diversity among household pets does occur . The latter was also seen in our results as dog P-853 had a different sequence type; ST71. It is possible that these dogs have acquired MRSP from a veterinary clinic where a family member worked. These dogs did not have apparent risk factors for MRSP carriage.
The results of the follow-up samples varied. Dog P-860, who had been exposed to the long term carrier P-843, was likely a transient carrier or perhaps even it was a case of contamination, as no positive samples were detected during follow-up. Windahl et al.  investigated the length of MRSP carriage and the factors that impacted this. Their results indicated that the median length of MRSP carriage is 11 months after clinically apparent infection, but that antimicrobial therapy with a drug to which the bacterium was resistant prolonged carriage time. The study used a cut off value of two consecutive MRSP-negative specimens to determine the dog was MRSP free. However, a longitudinal study of MRSP in households found that the results from MRSP screening varied in the individual dog . This was also the case in our study, as recurrent findings of MRSP in dogs that previously had two MRSP negative specimens (P-843 and P-853) were observed. It is noteworthy however that decolonization therapy was attempted in these cases. This may have altered the dynamics of the dogs’ microbiome causing unpredictable results. Saijonmaa-Koulumies et al.  studied the use of fusidic acid as a means to reduce the number of S. pseudintermedius (then S. intermedius) on mucous membranes of dogs. They found that while therapy did reduce the number of bacteria, the effect was only temporary. It would seem logical to think that MRSP behaved in the same fashion. S. pseudintermedius can however be a bacterium difficult to eradicate from dogs, as it is part of the normal microbiota and is carried by a majority of dogs . The use of decolonization therapy for MRSP is a subject of further study.
Based on our findings it seems that neither MRSA nor MRSP are a major concern in the GDS. The standard hygiene practices likely contribute to the low prevalence of MRSA and MRSP at the GDS’s kennels as they are likely higher than in most animal shelters. Also, the GDS’s kennels do not suffer from many problems one could see in rescue kennels, such as overcrowding or severely ill animals . It is however still advised that the GDS should routinely screen dogs with identifiable risk factors, such as skin or ear problems, or dogs that receive a lot of antimicrobials.
MRSP and MRSA have low prevalence and are not major problems among guide dogs in Finland. The MRSP population in these dogs is epidemiologically heterogeneous and there is little evidence of clonal spread. The number of antimicrobial courses and veterinary visits are identifiable risk factors for MRSP even in a low prevalence canine population.
Guide Dog School of the Finnish Federation of the Visually Impaired
multilocus sequence typing
methicillin resistant Staphylococcus aureus
methicillin resistant Staphylococcus pseudintermedius
pulsed-field gel electrophoresis
- SCCmec :
staphylococcal cassette chromosome mec
Paul NC, Bargman SC, Moodley A, Nielsen SS, Guardabassi L (2012) Staphylococcus pseudintermedius colonization patterns and strain diversity in healthy dogs: a cross-sectional and longitudinal study. Vet Microbiol 160:420–427
van Duijkeren E, Catry B, Greko C, Moreno MA, Pomba MC, Pyorala S et al (2011) Review on methicillin-resistant Staphylococcus pseudintermedius. J Antimicrob Chemother 66:2705–2714
Loeffler A, Linek M, Moodley A, Guardabassi L, Sung JM, Winkler M et al (2007) First report of multiresistant, mecA-positive Staphylococcus intermedius in Europe: 12 cases from a veterinary dermatology referral clinic in Germany. Vet Dermatol 18:412–421
Jones RD, Kania SA, Rohrbach BW, Frank LA, Bemis DA (2007) Prevalence of oxacillin- and multidrug-resistant staphylococci in clinical samples from dogs: 1,772 samples (2001-2005). J Am Vet Med Assoc 230:221–227
Perreten V, Kadlec K, Schwarz S, Gronlund Andersson U, Finn M, Greko C et al (2010) Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study. J Antimicrob Chemother 65:1145–1154
Borjesson S, Landen A, Bergstrom M, Andersson UG (2012) Methicillin-resistant Staphylococcus pseudintermedius in Sweden. Microb Drug Resist 18:597–603
Grönthal T, Moodley A, Nykäsenoja S, Junnila J, Guardabassi L, Thomson K et al (2014) Large Outbreak Caused by Methicillin Resistant Staphylococcus pseudintermedius ST71 in a Finnish Veterinary Teaching Hospital—from outbreak control to outbreak prevention. PLoS One 9:e110084
Boost MV, O’Donoghue MM, James A (2008) Prevalence of Staphylococcus aureus carriage among dogs and their owners. Epidemiol Infect 136:953–964
Rich M, Roberts L (2006) MRSA in companion animals. Vet Rec 159:535–536
van Duijkeren E, Kamphuis M, van der Mije IC, Laarhoven LM, Duim B, Wagenaar JA et al (2011) Transmission of methicillin-resistant Staphylococcus pseudintermedius between infected dogs and cats and contact pets, humans and the environment in households and veterinary clinics. Vet Microbiol 150:338–343
Gomez-Sanz E, Torres C, Ceballos S, Lozano C, Zarazaga M (2013) Clonal dynamics of nasal Staphylococcus aureus and Staphylococcus pseudintermedius in dog-owning household members. Detection of MSSA ST(398). PLoS One 8:e69337
Opaskoirakoulu | Näkövammaisten keskusliitto ry [http://www.opaskoirakoulu.fi/?lang=en]. Accessed 20 February 2015
Epi Tools—Sample size to estimate a proportion with specified precision. [http://epitools.ausvet.com.au/content.php?page=1Proportion]. Accessed 16 May 2015
Feng Y, Tian W, Lin D, Luo Q, Zhou Y, Yang T et al (2012) Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius in pets from South China. Vet Microbiol 160:517–524
Gingrich EN, Kurt T, Hyatt DR, Lappin MR, Ruch-Gallie R (2011) Prevalence of methicillin-resistant staphylococci in northern Colorado shelter animals. J Vet Diagn Invest 23:947–950
Griffeth GC, Morris DO, Abraham JL, Shofer FS, Rankin SC (2008) Screening for skin carriage of methicillin-resistant coagulase-positive staphylococci and Staphylococcus schleiferi in dogs with healthy and inflamed skin. Vet Dermatol 19:142–149
Hanselman BA, Kruth SA, Rousseau J, Weese JS (2009) Coagulase positive staphylococcal colonization of humans and their household pets. Can Vet J 50:954–958
Murphy C, Reid-Smith RJ, Prescott JF, Bonnett BN, Poppe C, Boerlin P et al (2009) Occurrence of antimicrobial resistant bacteria in healthy dogs and cats presented to private veterinary hospitals in southern Ontario: A preliminary study. Can Vet J 50:1047–1053
Sasaki T, Kikuchi K, Tanaka Y, Takahashi N, Kamata S, Hiramatsu K (2007) Methicillin-resistant Staphylococcus pseudintermedius in a veterinary teaching hospital. J Clin Microbiol 45:1118–1125
Vengust M, Anderson ME, Rousseau J, Weese JS (2006) Methicillin-resistant staphylococcal colonization in clinically normal dogs and horses in the community. Lett Appl Microbiol 43:602–606
Hanselman BA, Kruth S, Weese JS (2008) Methicillin-resistant staphylococcal colonization in dogs entering a veterinary teaching hospital. Vet Microbiol 126:277–281
Epi Tools—Calculate confidence limits for a sample proportion. [http://www.epitools.ausvet.com.au/content.php?page=CIProportion]. Accessed 15 March 2015
Brown LD, Cai TT, DasGupta A, Agresti A, Coull BA, Casella G et al (2001) Interval estimation for a binomial proportion—comment—rejoinder. Stat Sci 16:101–133
Firth D (1993) Bias reduction of maximum likelihood estimates. Biometrika 80:27–38
Venzon DJ, Moolgavkar SH (1988) A method for computing profile-likelihood-based confidence intervals. Appl Stat J Roy St C 37:87–94
Clinical and Laboratory Standards Institute (2013) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard, 4th edn. CLSI, Wayne
Clinical and Laboratory Standards Institute (2013) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; second informational supplement. VET01-S2. CLSI, Wayne
National Institute of Health and Welfare (2015) Vanha FiRe-standardi (the old FiRe-standard). https://www.thl.fi/fi/web/infektiotaudit/seuranta-ja-epidemiat/mikrobilaakeresistenssi/fire-suomalainen-mikrobilaakeresistenssin-tutkimusryhma/menetelmat/vanha-fire-standardi. Accessed 27 May 2015
Murchan S, Kaufmann ME, Deplano A, de Ryck R, Struelens M, Zinn CE et al (2003) Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41:1574–1585
DTU Food—National Food Institute (2012) Protocol for PCR-Amplification of mecA, mecC (mecA LGA251), spa and pvl Recommended by the EURL-AR, 2nd version. National Food Institute, Søborg
Stegger M, Andersen PS, Kearns A, Pichon B, Holmes MA, Edwards G et al (2012) Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecA(LGA251). Clin Microbiol Infect 18:395–400
Kondo Y, Ito T, Ma XX, Watanabe S, Kreiswirth BN, Etienne J, Hiramatsu K (2007) Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother 51:264–274
Solyman SM, Black CC, Duim B, Perreten V, van Duijkeren E, Wagenaar JA et al (2013) Multilocus sequence typing for characterization of Staphylococcus pseudintermedius. J Clin Microbiol 51:306–310
Staphylococcus pseudintermedius MLST Databases. [http://www.pubmlst.org/spseudintermedius/]. Accessed 16 March 2015
Lehner G, Linek M, Bond R, Lloyd DH, Prenger-Berninghoff E, Thom N et al (2014) Case-control risk factor study of methicillin-resistant Staphylococcus pseudintermedius (MRSP) infection in dogs and cats in Germany. Vet Microbiol 168:154–160
Nienhoff U, Kadlec K, Chaberny IF, Verspohl J, Gerlach GF, Kreienbrock L et al (2011) Methicillin-resistant Staphylococcus pseudintermedius among dogs admitted to a small animal hospital. Vet Microbiol 150:191–197
Soares Magalhaes RJ, Loeffler A, Lindsay J, Rich M, Roberts L, Smith H et al (2010) Risk factors for methicillin-resistant Staphylococcus aureus (MRSA) infection in dogs and cats: a case-control study. Vet Res 41:55
Shaw SC, Wood JL, Freeman J, Littlewood JD, Hannant D (2004) Estimation of heritability of atopic dermatitis in Labrador and Golden Retrievers. Am J Vet Res 65:1014–1020
Hellman JAO, Bengtsson B, Greko C (2013) SWEDRES-SVARM 2013. Use of antimicrobials and occurrence of antimicrobial resistance in Sweden. Solna/Uppsala, Sweden
Osland AM, Vestby LK, Fanuelsen H, Slettemeas JS, Sunde M (2012) Clonal diversity and biofilm-forming ability of methicillin-resistant Staphylococcus pseudintermedius. J Antimicrob Chemother 67:841–848
Perreten V, Chanchaithong P, Prapasarakul N, Rossano A, Blum SE, Elad D et al (2013) Novel pseudo-staphylococcal cassette chromosome mec element (psiSCCmec57395) in methicillin-resistant Staphylococcus pseudintermedius CC45. Antimicrob Agents Chemother 57:5509–5515
Laarhoven LM, de Heus P, van Luijn J, Duim B, Wagenaar JA et al (2011) Longitudinal study on methicillin-resistant Staphylococcus pseudintermedius in households. PLoS ONE 6:e27788
Siak M, Burrows AK, Coombs GW, Khazandi M, Abraham S, Norris JM et al (2014) Characterization of meticillin-resistant and meticillin-susceptible isolates of Staphylococcus pseudintermedius from cases of canine pyoderma in Australia. J Med Microbiol 63:1228–1233
Windahl U, Reimegard E, Holst BS, Egenvall A, Fernstrom L, Fredriksson M et al (2012) Carriage of methicillin-resistant Staphylococcus pseudintermedius in dogs–a longitudinal study. BMC Vet Res 8:34
Saijonmaa-Koulumies L, Parsons E, Lloyd DH (1998) Elimination of Staphylococcus intermedius in healthy dogs by topical treatment with fusidic acid. J Small Anim Pract 39:341–347
Miller L, Hurley K (2009) Infectious disease management in animal shelters, 1st edn. Wiley-Blackwell, Iowa
TG participated in the planning of the study, performed the mecA-PCR, assisted in MLST and SCCmec-typing, analyzed the data, performed microbiological testing and drafted the manuscript. MO participated in the planning of the study, data entry, sample culturing and drafted the manuscript. ME performed MLST typing, participated in PFGE typing, data entry, and drafted the manuscript. HP set up the molecular methods and performed microbiological testing. VG performed PFGE and SCCmec-typing and drafted the manuscript. JJ performed statistical analyses and interpretation. LS-K had the original idea for the study and participated in the drafting of the manuscript. RL participated in the planning of the study and provided samples and data. MR supervised the work, assisted in analyzing the data and in preparing the manuscript, and performed microbiological testing. All authors read and approved the final manuscript.
The researchers wish to thank the personnel of the Guide Dogs School for their participation and efforts in the making of this study; especially Vesa Väkeväinen and Eveliina Kumpulainen are acknowledged. We also thank Marja Matikka and Gina Meder for their excellent technical assistance.
Compliance with ethical guidelines
Competing interests The authors declare that they have no competing interests.
Gel image of the products of isolate P-781 and positive controls after SCCmec PCR. Description of data: ctr = control; SCCmec I ctr: Staphylococcus aureus NCTC 10442; SCCmec II ctr: Staphylococcus aureus ATCC BAA-1720; SCCmec III ctr: Staphylococcus aureus ATCC BAA-43; SCCmec V ctr: Staphylococcus aureus JCSC 6944; SCCmec VI ctr: Staphylococcus aureus ATCC BAA-42.
AscI PFGE dendogram of the MRSP-isolates investigated. Description of data: All isolates were also investigated using SmaI restriction (see text).
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Grönthal, T., Ollilainen, M., Eklund, M. et al. Epidemiology of methicillin resistant Staphylococcus pseudintermedius in guide dogs in Finland. Acta Vet Scand 57, 37 (2015). https://0-doi-org.brum.beds.ac.uk/10.1186/s13028-015-0129-8
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