Table 1 Findings in 13 puppies from 11 litters found positive for canid herpesvirus-1 by real-time polymerase chain reaction (PCR)
From: Prevalence of canid herpesvirus-1 infection in stillborn and dead neonatal puppies in Denmark
Litter no. | Puppy no. | Time of death (Days post partum) | PCR (Cp value) | Most significant lesions | In situ hybridization | |
|---|---|---|---|---|---|---|
Liver/lung | Spleen/kidney | |||||
1 | 1 | 13 | 13.3 | 13.9 | Acute disseminated necrosis in multiple tissues with intranuclear inclusion bodies | Strong labeling was detected in nuclei of cells in close proximity to focal areas of necrosis including hepatocytes, adrenal cortical cells, renal tubular epithelial cells, bronchial epithelial cells and pneumocytes. |
2 | 2 | 12 | 29.5 | 31.4 | Focal liver abscess. Mild macrocytic aspiration bronchopneumonia | A few ISH positive cells, most suggestive of macrophages based on morphology, were found in the liver, spleen and kidney |
3 | 3 | 2 | 32.8 | 32.6 | Mild macrocytic aspiration bronchopneumonia. Severe hepatic congestion. | Negative |
4 | 3 | 32.9 | 32.5 | Mild interstitial suppurative hepatitis | Negative | |
4 | 7 | 12 | 37.8 | 36.7 | Hepatomegaly. Widespread splenic single cell necrosis | Negative |
5 | 8 | 0 | 34.0 | NA | Severe autolysis. Inconclusive | ND |
6 | 11 | 17 | Negative | >40* | Hepatomegaly, splenomegaly and renomegaly. Alveolar hemorrhage and erythrophagocytosis | Negative |
7 | 12 | 0 | >40 | 39.4 | Severe autolysis. Inconclusive | ND |
13 | 0 | 34.8 | 36.9 | Severe autolysis. Inconclusive | Negative | |
8 | 15 | 0 | 35.7 | >40 | Polydactyli. Congenital atelectasis. Mild aspiration bronchopneumonia | Negative |
9 | 16 | 14 | 38.9 | Negative | Acute severe necrotizing and suppurative bronchopneumonia. Hepatomegaly and splenomegaly. | Negative |
10 | 17 | 1 | 33.6 | > 40 | Mild macrocytic bronchopneumonia | Negative |
11 | 18 | 14 | 31.3 | Negative | Acute severe suppurative bronchopneumonia. Widespread acute suppurative interstitial hepatitis. Hepatomegaly, splenomegaly and renomegaly. Follicular purulent dermatitis. | Negative |