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In Vitro Adherence of Staphylococcus Aureus to Bovine Mammary Gland Epithelial Cells

In vitro studier av Staphylococcus aureus adherens till bovina juverepitelceller

Abstract

A simple in vitro test system to study the adherence of bacteria to bovine mammary gland epithelial cells is described. This test system can be used as a laboratory model to study the relative attachment of bacterial species associated with mastitis. The effects of a number of physical, chemical, enzymatic and biological factors on the adherence of a strain of Staphylococcus aureus were studied using this test system. The numbers of bacteria adhering to epithelial cells were higher when logarithmic growth phase cultures were used (4–6 h). Adherence was not affected by pretreatment of bacteria with NaCl or EDTA, or within the pH range 6–8, but was inhibited by heat (60°C for 30 min) and trypsin and papain pretreatments, whereas wheat germ lipase pretreatment enhanced adherence. Sodium lauryl sulphate, Triton X-100, and Tween 80 inhibited adherence. Adherence was also inhibited by milk from a quarter infected with S. aureus and by specific anti-staphylococcal antiserum. These findings suggest that the staphylococcal adhesin may be protein in nature.

Sammanfattning

En enkel in vitro test för att studera bakteriers adherens (vidhäftning) till bovina juverepitelceller beskrives. Metoden kan användas för att pröva olika mastitframkallande bakteriers adhesionsförmåga. Undersökningar har gjorts för att klarlägga hur fysikaliska, kemiska, enzymatiska och biologiska faktorer påverkar vidhäftningsförmågan hos en Staphylococcus aureus stam. Antalet bakterier som „fastnade“ på epitelcellerna var högre när celler från aktivt växande bakteriekulturer (log-fas) användes. Vidhäftningen påverkades inte av en förbehandling av bakterierna med NaCl eller EDTA. Däremot minskades den om mikroberna upphettades tili 60°C under 30 min eller om de behandlades med trypsin eller papain. Vetegroddolja stimulerade adhesionen. Natrium lauryl sulfat, Triton X-100 och Tween 80 inhiberade. Specifikt antiserum inhiberade vidhäftningen liksom mjölk från juver infekterat med Staph. aureus.

References

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Wanasinghe, D.D. In Vitro Adherence of Staphylococcus Aureus to Bovine Mammary Gland Epithelial Cells. Acta Vet Scand 22, 99–108 (1981). https://0-doi-org.brum.beds.ac.uk/10.1186/BF03547211

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  • DOI: https://0-doi-org.brum.beds.ac.uk/10.1186/BF03547211

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