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Detection of Porcine Rotavirus by EM, ELISA and CIET

Påvisning af porcin rotavirus ved hjælp af EM, ELISA og CIET

Abstract

Feces samples from swine-herds with severe problems of neonatal diarrhoea, 3 weeks scours or early weaning diarrhoea were examined for porcine rotavirus using three techniques: EM, ELISA and CIET. Infection with rotavirus was found in about one third of the feces samples, representing more than half of the examined swine-herds. EM and ELISA revealed nearly all the samples found to be positive, while CIET as used here detected only half of them.

Sammendrag

Fæcesprøver fra svinebesætninger med længere varende problemer med neonatal, tre ugers eller tidlig fravænnings diarré blev undersøgt for tilstedevserelse af porcin rotavirus ved hjælp af elektronmikroskopi (EM), “enzyme linked immunosorbent assay” (ELISA), og modstrømselektroforese (CIET). Rotavirus blev påvist i omkring en trediedel af fæcesprøverne og i mere end halvdelen af de undersøgte svinebesætninger. I næsten alle rotavirus-positive prøver fandtes virus ved hjælp af EM og ELISA, mens det kun var muligt at påvise virus i halvdelen af alle rotavirus-positive prøver ved hjælp af CIET.

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This work was supported by the Danish Agricultural and Veterinary Research Council.

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Askaa, J., Bloch, B. Detection of Porcine Rotavirus by EM, ELISA and CIET. Acta Vet Scand 22, 32–38 (1981). https://0-doi-org.brum.beds.ac.uk/10.1186/BF03547203

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