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Table 1 Antibodies used for immunohistochemistry on paraffin and/or frozen sections

From: Chronic pneumonia in calves after experimental infection with Mycoplasma bovis strain 1067: Characterization of lung pathology, persistence of variable surface protein antigens and local immune response

Antibody
Clone/designation1
Working dilution2 Type or isotype Specificity3 Antigen retrieval4 Source/Reference
mAbpool 1:2000a Mouse IgG1 and IgM M. bovis 0.25% trypsin (37°C, 60 min) Chemicon, Temecula, CA, USA
1A1 1:200a/1:800b Mouse IgG1 M. bovis Vspa A, C 0.25% trypsin (37°C, 60 min) [46]
1E5 u.d.c/1:70b Mouse IgM M. bovis Vspa A, B, C 0.25% trypsin (37°C, 60 min) [47]
I2 1:900b Mouse IgG1 M. bovis pMB67b w/oa [7, 48]
CC30 1:5b Mouse IgG1 Bovine CD4 w/oa Biozol, Eching, Germany
CC63 1:50b Mouse IgG2a Bovine CD8 w/oa Biozol, Eching, Germany
A10-116Fa 1:8000a Sheep IgG Bovine IgG1 0.05% pronase E (37°C, 20 min) Bethyl Laboratories, Montgomery, TX, USA
A10-117a 1:3000a Sheep IgG Bovine IgG2 0.05% pronase E (37°C, 20 min) Bethyl Laboratories, Montgomery, TX, USA
A10-100Fa 1:400a Rabbit IgG Bovine IgM 0.05% pronase E (37°C, 20 min) Bethyl Laboratories, Montgomery, TX, US
A10-108Fa 1:4000a Rabbit IgG Bovine IgA 0.05% pronase E (37°C, 20 min) Bethyl Laboratories, Montgomery, TX, USA
AE1/AE3 1:500a Mouse IgG Wide spectrum-cytokeratin 0.05% pronase E (37°C, 20 min) DakoCytomation, Hamburg, Germany
TAL.1B5 1:4000a Mouse IgG1 α-chain of human leukocyte antigen (HLA-DR) 0.01 M citric buffer (microwave 95°C, 15 min) DakoCytomation, Hamburg, Germany
  1. 1a Conjugated to fluorescein isothiocyanate (FITC).
  2. 2a On paraffin sections. 2b On frozen sections. c Antibody was used undiluted.
  3. 3a Variable surface membrane protein of M. bovis. b Non-Vsp surface antigen of M. bovis.
  4. 4a Without antigen retrieval.