Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen

Table 1 RT-PCR results of PCV2-infected foetuses and gilts

		Number foetuses	Serum titre against PCV2*	Viremia DAI**	Tonsil	Left mesometrial lymph nodes	Right mesometrial lymph nodes	Tracheo- bronchial lymph node	Cervix	Foetuses (pos/tot.)	Amniotic fluid (pos/tot.)	Foetus and corresponding endometrium (left)	Foetus and corresponding endometrium (right)
Infected pregnant gilts	I1	16	1/10²	21 and 35	-	+	+	+	-	10^§/16	-	8***	1
	I2	11	1/10³	-	-	-	-	-	-	-	1/11	-	-
	I4	7	1/10³	21	-	-	-	-	-	3/7	-	-	-
Infected non- pregnant gilts	I3	0	1/10⁴	7	+	-	+	n.a.	-	-	/	-	-
	I5	0	1/10⁴	28	-	-	-	-	+	-	/	-	-
	I6	0	1/10³	-	-	-	-	n.a.	-	-	/	+	-
Control gilts	C1	11	1/10³	21 and 35^§§	-	-	-	-	-	-	-	-	-
Control gilts	C2	0	1/10⁴	-	-	-	-	-	-	-	/	-	-
	C3	8	1/10⁴	-	-	-	-	-	-	-	-	-	-

Notes:
“+” PCR-positive sample,
“-“ PCR-negative sample.
“n.a.” sample not-available.
* serum titres at the beginning of the experimental study.
** Days post artificial insemination/s of sampling when viraemia was recorded.
*** out of the 16 uterine tract samples, each corresponding to a foetus, 8 were PCV2 positive by PCR, but only in 7 cases there was concordance of the positive PCR result in both foetus and corresponding endometrium.
^§ a foetus and its placenta were IHC positive, as well.
^§§ both of them are PCV2a, strain not-used for the infection.

ISSN: 1751-0147