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Table 1 Back titrations (in TCID 50 /well) and average titres of positive (n = 6) and negative (n = 2) samples, depending on intended TCID 50 per well (100 vs. 500) and days of incubation of the VNT (3, 4, or 5 days)

From: Development of a virus neutralisation test to detect antibodies against Schmallenberg virus and serological results in suspect and infected herds

 

100 TCID50

500 TCID50

  

3 days

4 days

5 days

3 days

4 days

5 days

Virus titres

Back titration

Microsc

59

154

202

314

720

1389

AmidoB

7

41

56

35

187

562

Antibody titres

Positive samples

Microsc

211

215

181

78

57

51

AmidoB

2048

813

287

575

228

181

Negative samples

Microsc

3

4

3

3

2

3

 

AmidoB

16

6

3

6

4

3

  1. Plates were read in two ways: microscopic (Microsc), where CPE was defined as even a single plaque of CPE, and with amido black staining (AmidoB), where CPE was defined as 75 % or more of the monolayer gone (is <25% staining).
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Acta Veterinaria Scandinavica

ISSN: 1751-0147