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Table 1 Back titrations (in TCID 50 /well) and average titres of positive (n = 6) and negative (n = 2) samples, depending on intended TCID 50 per well (100 vs. 500) and days of incubation of the VNT (3, 4, or 5 days)

From: Development of a virus neutralisation test to detect antibodies against Schmallenberg virus and serological results in suspect and infected herds

  100 TCID50 500 TCID50
   3 days 4 days 5 days 3 days 4 days 5 days
Virus titres
Back titration Microsc 59 154 202 314 720 1389
AmidoB 7 41 56 35 187 562
Antibody titres
Positive samples Microsc 211 215 181 78 57 51
AmidoB 2048 813 287 575 228 181
Negative samples Microsc 3 4 3 3 2 3
  AmidoB 16 6 3 6 4 3
  1. Plates were read in two ways: microscopic (Microsc), where CPE was defined as even a single plaque of CPE, and with amido black staining (AmidoB), where CPE was defined as 75 % or more of the monolayer gone (is <25% staining).