Figure 1

Gel electrophoresis of chOPG. 1A: Gel electrophoresis of reverse transcription polymerase chain reaction (RT-PCR) product. Total RNA extracted from chicken embryo frontal bone was analyzed using RT-PCR with specific primers. About 1.2 kbp gene of chicken osteoprotegerin (chOPG) was amplified (lane 1); DL2000 marker (lane 2); 1B: Gel electrophoresis of pcDNA3.1 (+)/chOPG PCR product. chOPG fragment was inserted into the eucaryon expression vector pcDNA3.1 (+) between Nhe| and Xho|. Negative plasmid (lane 1) and positive plasmid (lane 2) were chosen using PCR; marker (lane 3); 1C: Gel electrophoresis of pcDNA3.1 (+)/chOPG double restriction enzyme assay. Positive plasmid (lane 3) was identified by Nhe|and Xho|double restriction digestion and showed pcDNA3.1 (+) and OPG (lane 2); marker (lane 1); 1D: Gel electrophoresis of RT-PCR analysis showing the expression of chOPG gene at 48 h. Amplification of chOPG using cDNA from lane 1 (control group) and lane 2 (pcDNA3.1 (+) transfected CEFs group) showing negative result. Amplification of chOPG using cDNA from lane 4 (pcDNA3.1 (+)/chOPG transfected chicken embryo fibroblasts group) showing about 1200 bp gene of chOPG. Lane 3: marker