Isolation and molecular characterization of multidrug-resistant Gram-negative bacteria from imported flamingos in Japan

Table 1 Primers used in this study

Primer	Sequence (5' to 3')	Target	Reference
Integrons
5'-CS 3'-CS	GGCATCCAAGCAGCAAG AAGCAGACTTGACCTGA	Class 1 integron	11
hep74 hep51	CGGGATCCCGGACGGCATGCACGATTTGTA GATGCCATCGCAAGTACGAG	Class 2 integron	11
β-Lactamases
TEM-F TEM-R	ATAAAATTCTTGAAGACGAAA GACAGTTACCAATGCTTAATC	bla _TEM	11
SHV-F SHV-R	TT ATCTCCCTGTTAGCCACC GATTTGCTGATTTCGCTCGG	bla _SHV	11
OXA-F OXA-R	TCAACTTTCAAGATCGCA GTGTGTTTAGAATGGTGA	bla _OXA	11
CTX-M-F CTX-M-R	CGCTTTGCGATGTGCAG ACCGCGATATCGTTGGT	bla _CTX-M	11
CTX-M-F2 CTX-M-R2	CCAGAATAAGGAATCCCATG GCCGTCTAAGGCGATAAAC	whole bla_CTX-M	11
CMY-F CMY-R	GACAGCCTCTTTCTCCACA TGGAACGAAGGCTACGTA	bla _CMY	11
Plasmid-mediated quiniolone resistance
qnrA-F qnrA-R	ATTTCTCACGCCAGGATTTG GATCGGCAAAGGTTAGGTCA	qnrA	19
qnrB-F qnrB-R	GATCGTGAAAGCCAGAAAGG ACGATGCCTGGTAGTTGTCC	qnrB	19
qnrS-F qnrS-R	ACGACATTCGTCAACTGCAA TAAATTGGCACCCTGTAGGC	qnrS	19

ISSN: 1751-0147