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Figure 2 | Acta Veterinaria Scandinavica

Figure 2

From: Commercially Available Antibodies to Human Tumour Necrosis Factor-α Tested for Cross-Reactivity with Ovine and Bovine Tumour Necrosis Factor-α using Flow Cytometric Assays

Figure 2

Standard curves of 2 different recombinant human TNF-α analysed with the Fluorokine® MAP-kit, TNF-α from Serotec Ltd (-■-) and TNF-α from R&D Systems (--). Curves are presented as mean fluorescence intensity versus concentration of standards. Results represent the mean (SD) of 4 standards performed in duplicate in 2 different experiments. The limit of detection for the assay is shown as a dotted line and was defined as the concentration corresponding to the signal 3 SD above the mean fluorescence of 4 samples without TNF-α. The mean fluorescence of the samples without TNF-α is shown as a dashed line.

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